ΔG°′ | ||||||
|---|---|---|---|---|---|---|
Step | Reaction | Enzyme | Prosthetic group | Type* | kJ mol−1 | kcal mol−1 |
1 | Acetyl CoA + oxaloacetate + H2O → citrate + CoA + H+ | Citrate synthase | a | –31.4 | –7.5 | |
2a | Aconitase | F | b | +8.4 | +2.0 | |
2b | Aconitase | F | c | −2.1 | −0.5 | |
3 | Isocitrate dehydrogenase | d + e | −8.4 | −2.0 | ||
4 | α-Ketoglutarate dehydrogenase complex | Lipoic acid, FAD, TPP | d + e | −30.1 | −7.2 | |
5 | Succinyl CoA synthetase | f | −3.3 | −0.8 | ||
6 | Succinate dehydrogenase | FAD, F | e | 0 | 0 | |
7 | Fumarase | c | −3.8 | −0.9 | ||
8 | Malate dehydrogenase |
| e | +29.7 | +7.1 | |
*Reaction type: (a) condensation; (b) dehydration; (c) hydration; (d) decarboxylation; (e) oxidation; (f) substrat | ||||||