Proteomic analysis by mass spectrometry. This mass spectrum was obtained by analyzing a trypsin-treated band in a gel derived from a yeast nuclear-pore sample. Many of the peaks were found to match the masses predicted for peptide fragments from three proteins (Nup120p, Kap122p, and Kap120p) within the yeast genome. The band corresponded to an apparent molecular mass of 100 kDa.

[Data from M. P. Rout, J. D. Aitchison, A. Suprapto, K. Hjertaas, Y. Zhao, and B. T. Chait. J. Cell Biol. 148:635–651, 2000.]