Solid-phase peptide synthesis. The sequence of steps in solid-phase synthesis is: (1) anchoring of the C-terminal amino acid to a solid resin, (2) deprotection of the amino terminus, and (3) coupling of the free amino terminus with the DCC-activated carboxyl group of the next amino acid. Steps 2 and 3 are repeated for each added amino acid. Finally, in step 4, the completed peptide is released from the resin.