3.2 Preparing Monoclonal Antibodies
A(n) , or antibody generator, is any substance that elicits an immune response in a host animal.
are agents that boost the immune response to an antigen.
antibodies recognize multiple sites on an antigen, whereas antibodies only recognize a single site on an antigen.
A is formed by fusing an antibody-producing B cell with a myeloma cell that is selected for its ability to grow in tissue culture.
True or False: A single exposure of a mouse to an antigen is usually sufficient to generate a strong immunogenic response.
A. |
B. |
True or False: Myeloma cells are immortal and will grow indefinitely in tissue culture.
A. |
B. |
True or False: Only the myeloma cells have the ability to survive in HAT medium because they are HGPRT-.
A. |
B. |
You are a researcher and would like to make monoclonal antibodies for a new protein that you have discovered, protein Y, so that you can study the function and expression patterns of the protein. Click on the step and the associated process to identify the correct order for generating your monoclonal antibodies.
Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 | Add adjuvant to protein Y. Mix antibody-producing spleen cells with an HGPRT- myeloma cell line to generate hybridoma cells. Plate hybridomas in limiting dilution in HAT selection medium. Harvest spleen cells from mouse host. Isolate and purify protein Y. Inject adjuvant/protein Y mixture into a mouse host days at 0, 14, and 21. Isolate and test monoclonal antibodies generated by clonal hybridoma populations. |
Correct Matches: |
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