Chapter 5. 5.2 PCR

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5.2 PCR

1.

shared the Nobel Prize in 1993 for his work developing the technique of polymerase chain reaction.

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2.

A series of heating and cooling reactions are called .

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3.

List the five reagents that are critical for performing a PCR reaction:

_feedback: The five reagents are: Mg2+-based buffer, dNTPs, DNA primers, DNA template, and DNA polymerase.

4.

True or False: DNA polymerase requires Mg2+ as a cofactor for its reaction mechanism.

A.
B.

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5.

True or False: Taq polymerase is a heat-stable DNA polymerase that can survive sequential thermocycle rounds.

A.
B.

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6.

True or False: DNA primers that are used for PCR are usually long oligonucleotides that have melting temperatures of 95°C or greater.

A.
B.

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7.

True or False: As PCR thermocycles progress, the concentration of amplified DNA increases linearly throughout the run.

A.
B.

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Question 1 of 3

8.

Which thermocycle step is pictured in the diagram below?

thermocycle step diagram

A.
B.
C.

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