Chapter 5. 5.3 Plasmid Cloning

Introduction

Assessments for Animated Techniques

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5.3 Plasmid Cloning

Question 5.1

A vOjDHRD1G3XmcbzB is a circular extrachromosomal DNA molecule that can replicate independently from the host’s chromosomal DNA.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.2

Within a plasmid, the +3l3ItStaM9kjooXn7QdPgSKtOyp06i1kl4qu+52Yn0a/lpgf2honK1H6//Mb3pe has been engineered to contain many restriction enzyme sequences.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.3

Plasmids expressed in bacterial strains typically contain a(n) DITyYCOU2LQT56TL4iq4iWL5GSrpRPYw39kVXrtOtDM= that allows for the selective growth of bacteria that have been transformed with the plasmid.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.4

Within a plasmid, the DZl3G5ekRnKYBFSuan6uyEeyD0WyqADZlN2suw== allows for the plasmid to replicate within the host cell.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.5

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Question 5.6

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.7

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.8

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.9

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_feedback: Sticky ends have overlapping sequences between the plasmid DNA and the new fragment DNA that will be inserted into the plasmid. This creates intermolecular interactions stabilizing the structure for the DNA ligase reaction that will form the permanent covalent linkages.

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