Chapter 5. 5.4 Site-Directed Mutagenesis

Introduction

Assessments for Animated Techniques

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5.4 Site-Directed Mutagenesis

Question 5.1

In site-directed mutagenesis, the E8u09hbb6YpKAUAfqJXw6BWN/r0= enzyme is used to synthesize the mutant DNA strand from a single-stranded DNA template.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.2

A specific 4ZiSmwlEdGi1uUQGKXOyNzUUV57XvGgu, such as NciI, is used to selectively nick the DNA template strand while leaving the newly synthesized mutant strand intact.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.3

Next, qJzVDxExruOwwmPrkPbllKCxLZg= is used to progressively digest the 3’ end of the nicked DNA template strand.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.4

The gap created by exonuclease III is filled in using the E8u09hbb6YpKAUAfqJXw6BWN/r0= enzyme and the DNA backbone is resealed by wsqgtFECqzKoaL/s/7jvyw==.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.5

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.6

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.7

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 5.8

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_feedback: In this example, the restriction enzyme NciI is being used to selectively nick the template strand of DNA while leaving the newly synthesized DNA strand intact. It can do this because it is unable to recognize the modified sulfur-containing cytosine residue that is present within the mutant DNA strand.

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