5.5 Screening an Oligonucleotide
DNA is a copy of mRNA that is created by a reverse transcriptase enzyme.
The process of , or hydrogen bonding between DNA molecules, is used to anneal cDNA to the oligonucleotide probes on the microarray.
A is a chemical compound that can re-emit light upon light excitation.
The enzyme is produced by retroviruses to synthesize DNA from an RNA template.
True or False: Once isolated from a desired source, mRNA can be used directly to screen a microarray.
A. |
B. |
True or False: When screening the DNA microarray, it is important to remember that the oligonucleotide probes contain a fluorescent label that allows easy detection.
A. |
B. |
True or False: Microarrays can be screened by two different sets of cDNA molecules as long as each contains a different colored fluorophore tag.
A. |
B. |
G-gene expression in a normal cell population is being compared with gene expression in a tumor cell line, and the normal cell cDNA is labeled with a green fluorophore while the tumor cell cDNA is labeled with a red fluorophore. Match the following conditions with their array profile (labels A-C on the diagram).
A B C | Transcripts that are only expressed in the normal cell type Transcripts that are expressed in both cell types Transcripts that are only expressed in the tumor cells |
Correct Matches: |
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