Chapter 5. 5.5 Screening an Oligonucleotide

Assessments for Animated Techniques
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5.5 Screening an Oligonucleotide

1.

DNA is a copy of mRNA that is created by a reverse transcriptase enzyme.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

2.

The process of , or hydrogen bonding between DNA molecules, is used to anneal cDNA to the oligonucleotide probes on the microarray.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

3.

A is a chemical compound that can re-emit light upon light excitation.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

4.

The enzyme is produced by retroviruses to synthesize DNA from an RNA template.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

5.

True or False: Once isolated from a desired source, mRNA can be used directly to screen a microarray.

A.
B.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

6.

True or False: When screening the DNA microarray, it is important to remember that the oligonucleotide probes contain a fluorescent label that allows easy detection.

A.
B.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

7.

True or False: Microarrays can be screened by two different sets of cDNA molecules as long as each contains a different colored fluorophore tag.

A.
B.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

8.

G-gene expression in a normal cell population is being compared with gene expression in a tumor cell line, and the normal cell cDNA is labeled with a green fluorophore while the tumor cell cDNA is labeled with a red fluorophore. Match the following conditions with their array profile (labels A-C on the diagram).

A
B
C
Transcripts that are only expressed in the normal cell type
Transcripts that are expressed in both cell types
Transcripts that are only expressed in the tumor cells
_feedback_correct: Correct. _feedback_incorrect: Incorrect.

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