Chapter 5. 5.7 Creating a Transgenic Mouse

Assessments for Animated Techniques
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5.7 Creating a Transgenic Mouse

1.

When a mouse egg cell or is fertilized by a sperm, the genetic material from the sperm will remain separate from the genetic material of the egg cell for several hours, forming two .

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2.

A is formed when two gamete cells (egg and sperm) are joined by means of sexual reproduction.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

3.

In the generation of a transgenic animal, the DNA is randomly integrated into a chromosome of the diploid zygote by the process of recombination.

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4.

True or False: Introduction of foreign DNA into a fertilized egg cell is usually done after the two pronuclei have fused together.

A.
B.

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5.

True or False: The gene of interest must be integrated into the host chromosome of the zygote prior to the first cell division, to ensure that all of the cells of the developing mouse contain the introduced gene of interest.

A.
B.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

6.

True or False: In the process of making a transgenic mouse, only 10% to 30% of the progeny will contain the transgene in all the tissues.

A.
B.

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7.

True or False: To study the full effects of the transgene, typically only one transgenic line will be developed and studied.

A.
B.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

8.

In the development of transgenic animals, resulting progeny are genotyped using which of these methodologies?

A.
B.
C.
D.
E.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

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