Chapter 41. 41.1 Dideoxy Sequencing of DNA, Polymerase Chain Reaction

Introduction

Assessments for Animated Techniques
true
false

41.1 Dideoxy Sequencing of DNA, Polymerase Chain Reaction

Question 41.1

OHYLiugqP1YjUIzNW52ibpdNOFVi3b6AHW5b6A== received a second Nobel Prize in 1980 for his work in the development of the dideoxy method of DNA sequencing.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.2

E8u09hbb6YpKAUAfqJXw6BWN/r0= is the enzyme required for the in vitro DNA sequencing reaction.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.3

A total of V3Ck19dPJ+RkxzRS sequencing reactions are required to generate the sequence for one DNA template.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.4

The DNA sequence that is closest to the DNA primer being used will be the gUHrDMev01uqGt24MZj1W7/3RncL/lHN fragment and will run near the eRNMgWmZzew/XyFRKYW4qg== of the gel.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.5

F66AoKZWBxvHL7NT6GHPTCJ+AJvcxDQVkeS//GjXDrjfQpULUW6lWEdXjn2pnKPPnWYdsxD8QNHl/UiucoDNUaS4+mAym4jVFsxhq/uzG8MLrnZVGyOTz8PVTrV8ZVuxhW43vAsNWss0AIOriB+UdZOcIDjYKzVL1HffutCBJXF4NMn5QYmZC3bMVUiYcUz1zrAWLNotJ0ClcWlDIqLwh2TqQ29YxbZuKGhTLpY19a9dni3+TcozDY8TVM+AwonaBGO7SgnlkEr9BhHp/F0BdZ88D4A=
_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.6

hfhKxDp71tRUy3K+ij0IFewm2fQazbZLDL9sFM4bgN6t9NqO3k4fXmloESlA5PZHb1WoW42CFgysprqJoX1yir+/mfS30Zjk8yaRKw9J5JxMmNh/9rhlJS1wSMwql2TZGOa5/coXOFbZJvuS
_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.7

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
_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.8

A patient is concerned that she may be a carrier for a hereditary disease. A DNA sample is taken from the patient and the gene of interest is sequenced. The wild-type gene is sequenced side by side for comparison.

Wild-Type Sequence:
5’ – ACTTCTTGGCAAGTAGGTTAAGATC – 3’

Patient X Sequence:
5’ – ACTTCTTGGCAAGTCGGTTAAGATC – 3’

DNA sample from Patient X, side-by-side with Wild-Type DNA sample
aZo6VtULiMqIPEY6JAjrWeLH29+JLq/t55n10/hYgo2sGqq5wNJ8AIiqosvb5iZuWCyht4xqTVOqVBVJOobgG3xbIWHGf092

The patient’s sequence shows both a G and C at position 16, indicating that she is heterozygous for this gene locus, carrying one wild-type allele and one mutant allele. She should be advised that she is a carrier for the disease trait.

Activity results are being submitted...