Introduction
Assessments for Animated Techniques
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41.2 Polymerase Chain Reaction
3.
List the five reagents that are critical for performing a PCR reaction:
_feedback: The five reagents are: Mg2+-based buffer, dNTPs, DNA primers, DNA template, and DNA polymerase.
4.
True or False: DNA polymerase requires Mg2+ as a cofactor for its reaction mechanism.
_feedback_correct: Correct.
_feedback_incorrect: Incorrect.
5.
True or False: Taq polymerase is a heat-stable DNA polymerase that can survive sequential thermocycle rounds.
_feedback_correct: Correct.
_feedback_incorrect: Incorrect.
6.
True or False: DNA primers that are used for PCR are usually long oligonucleotides that have melting temperatures of 95°C or greater.
_feedback_correct: Correct.
_feedback_incorrect: Incorrect.
7.
True or False: As PCR thermocycles progress, the concentration of amplified DNA increases linearly throughout the run.
_feedback_correct: Correct.
_feedback_incorrect: Incorrect.
Question 1 of 3
8.
Which thermocycle step is pictured in the diagram below?
_feedback_correct: Correct.
_feedback_incorrect: Incorrect.
9.
Which thermocycle step is pictured in the diagram below?
_feedback_correct: Correct.
_feedback_incorrect: Incorrect.
10.
Which thermocycle step is pictured in the diagram below?
_feedback_correct: Correct.
_feedback_incorrect: Incorrect.
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