Chapter 41. 41.3 Plasmid Cloning

Assessments for Animated Techniques
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41.3 Plasmid Cloning

1.

A is a circular extrachromosomal DNA molecule that can replicate independently from the host’s chromosomal DNA.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

2.

Within a plasmid, the has been engineered to contain many restriction enzyme sequences.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

3.

Plasmids expressed in bacterial strains typically contain a(n) that allows for the selective growth of bacteria that have been transformed with the plasmid.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

4.

Within a plasmid, the allows for the plasmid to replicate within the host cell.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

5.

is the process used to introduce the plasmid DNA into a bacterial host.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

6.

True or False: Due to its small size, plasmid DNA can easily be isolated away from chromosomal DNA.

A.
B.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

7.

True or False: Cells within a colony all contain copies of the same recombinant plasmid with its fragment of foreign DNA.

A.
B.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

8.

True or False: Daughter cells that receive a copy of the plasmid will become antibiotic sensitive and die in the presence of the antibiotic.

A.
B.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

9.

Why are restriction enzymes that create sticky ends more useful when inserting a DNA sequence into a plasmid than restriction enzymes that create blunt ends?

_feedback: Sticky ends have overlapping sequences between the plasmid DNA and the new fragment DNA that will be inserted into the plasmid. This creates intermolecular interactions stabilizing the structure for the DNA ligase reaction that will form the permanent covalent linkages.

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