Chapter 41. 41.3 Plasmid Cloning

Introduction

Assessments for Animated Techniques

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41.3 Plasmid Cloning

Question 41.1

A vOjDHRD1G3XmcbzB is a circular extrachromosomal DNA molecule that can replicate independently from the host’s chromosomal DNA.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.2

Within a plasmid, the +3l3ItStaM9kjooXn7QdPgSKtOyp06i1kl4qu+52Yn0a/lpgf2honK1H6//Mb3pe has been engineered to contain many restriction enzyme sequences.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.3

Plasmids expressed in bacterial strains typically contain a(n) DITyYCOU2LQT56TL4iq4iWL5GSrpRPYw39kVXrtOtDM= that allows for the selective growth of bacteria that have been transformed with the plasmid.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.4

Within a plasmid, the DZl3G5ekRnKYBFSuan6uyEeyD0WyqADZlN2suw== allows for the plasmid to replicate within the host cell.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.5

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.6

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.7

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.8

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 41.9

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_feedback: Sticky ends have overlapping sequences between the plasmid DNA and the new fragment DNA that will be inserted into the plasmid. This creates intermolecular interactions stabilizing the structure for the DNA ligase reaction that will form the permanent covalent linkages.

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