Chapter 41. 41.4 Screening an Oligonucleotide Array for Patterns of Gene Expression
Introduction
41.4 Screening an Oligonucleotide Array for Patterns of Gene Expression
1.
DNA is a copy of mRNA that is created by a reverse transcriptase enzyme.
2.
The process of , or hydrogen bonding between DNA molecules, is used to anneal cDNA to the oligonucleotide probes on the microarray.
3.
A is a chemical compound that can re-emit light upon light excitation.
4.
The enzyme is produced by retroviruses to synthesize DNA from an RNA template.
5.
True or False: Once isolated from a desired source, mRNA can be used directly to screen a microarray.
| A. |
| B. |
6.
True or False: When screening the DNA microarray, it is important to remember that the oligonucleotide probes contain a fluorescent label that allows easy detection.
| A. |
| B. |
7.
True or False: Microarrays can be screened by two different sets of cDNA molecules as long as each contains a different colored fluorophore tag.
| A. |
| B. |
8.
G-gene expression in a normal cell population is being compared with gene expression in a tumor cell line, and the normal cell cDNA is labeled with a green fluorophore while the tumor cell cDNA is labeled with a red fluorophore. Match the following conditions with their array profile (labels A-C on the diagram).
A B C | Transcripts that are only expressed in the tumor cells Transcripts that are only expressed in the normal cell type Transcripts that are expressed in both cell types |
Activity results are being submitted...