Chapter 41. 41.5 Synthesizing an Oligonucleotide Array

Assessments for Animated Techniques
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41.5 Synthesizing an Oligonucleotide Array

1.

The represents the gene expression patterns of a specific cell type at a given time.

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2.

In the DNA microarray methodology, small oligonucleotides are chemically synthesized and are then attached to a very fine grid using a robotic printing device.

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3.

In the DNA microarray methodology, oligonucleotides are synthesized directly on the microarray chip using UV-masking and light-directed combinatorial synthesis.

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4.

True or False: It is possible to use in-situ synthesized DNA microarray technologies to produce arrays with 65,000 to 100,000 oligonucleotides.

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B.

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5.

True or False: Each nucleotide added to the array needs to be protected so that unwanted extension of the oligonucleotide does not occur.

A.
B.

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6.

True or False: Once a mask is placed on the array, the protecting groups of the unmasked cells are removed with a chemical lysing reagent.

A.
B.

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7.

True or False: Arrays containing longer oligonucleotides allow for more accurate and specific identification of gene products.

A.
B.

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8.

Click on the step and the associated process to identify the series of steps required to construct an in-situ synthesized DNA microarray.

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A single nucleotide that contains a blocking group on the 3’-OH is added to the array with a cross-linking reagent.
The protecting group is removed from the unmasked cells by exposing the array to UV light.
The array is coated with a protecting group that covalently binds and blocks the linker.
The microarray is developed to contain a covalent linker that will dock the DNA oligonucleotide to the array.
A new mask is placed on the array and the cycle is repeated until oligonucleotides of the desired length are generated.
Unbound nucleotides are washed away from the array.
A mask is placed on the array.
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