You analyze a cell sample by both Southern and northern blotting for gene X. A band corresponding to gene X appeared via Southern blot, but the northern blot failed to detect a band. Which of the following could explain these results?
Huntington’s Disease (HD) is a dominant disease, meaning that a single copy of the mutated allele will lead to disease. Patients with HD have expanded numbers of a CAG repeat in the Huntington gene. You’d like to determine if a person has HD, and you run molecular testing on the Huntington gene and protein. Which of the following results would indicate that this person is healthy and unaffected by HD?
Diane is attempting to clone gene Y into a vector for protein expression. Following cloning, both her Southern and northern blots detect a fragment of the correct size, and her anti-protein Y antibody detects a band at the correct molecular weight via western blotting. Which of the following is NOT safe to assume?
