Chapter 9. Chapter 9

Problem-Solving Video Assessment

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Chapter 9 Problem-Solving Video Assessment

1.

What is the catalytic power of subtilisin after mutation of all three residues in the catalytic triad to alanine?

5.6 x 10¹²

1.7 x 10⁶

1.8 x 10³

0.59

5.6 x 10^-4

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

2.

Imagine that scientists identify a novel residue in the active site of subtilisin that is critical for enzyme activity. After mutating the residue to alanine, the scientists determine that the catalytic power of the mutant subtilisin is reduced 450-fold compared to wild-type enzyme (wild-type catalytic power = 3 x 10⁹). What is the rate of catalysis for the mutant subtilisin?

6.7 x 10¹⁴ s^-1

30.0 s^-1

6.7 x 10^-2 s^-1

4.5 x 10^-6 s^-1

1.5 x 10^-15 s^-1

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

3.

What is the catalytic power of carbonic anhydrase at pH 8?

6.3 x 10⁶

1.4 x 10⁵

1.9

0.53

1.6 x 10^-7

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

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Chapter 9. Chapter 9

Introduction

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