Chapter 14. Chapter 14

Introduction

Problem-Solving Video Assessment
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Chapter 14 Problem-Solving Video Assessment

You have discovered a new cell-surface receptor (receptorX) that you believe binds to either insulin or epidermal growth factor (EGF). To differentiate between the two ligands, you radiolabel each ligand to a specific activity of 4 x 107 cpm per micromole. You then perform ligand–receptorX binding experiments in your cell line and generate the following counts of cell-bound radioactivity:

insulin EGF
Radioactive ligand alone 5563 cpm 6012 cpm
Radioactive ligand + 1000-fold excess unlabeled ligand 237 cpm 5874 cpm
Table

Question 14.1

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 14.2

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 14.3

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 14.4

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

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