Chapter 28. Chapter 28

Problem-Solving Video Assessment

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Chapter 28 Problem-Solving Video Assessment

1.

Suppose that the entirety of E. coli DNA replication is completed in a medium containing highly radioactive tritiated thymidine. Which of the autoradiographic patterns below should result? The open circles and arrows represent the origin location and direction of replication, respectively.

Autoradiographic patterns 1-5, showing open circles and arrows representing the origin location and direction of replication, respectively

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

2.

The following five steps comprise a general overview of DNA replication in both prokaryotic and eukaryotic organisms. Which step is the second step in the process?

Okazaki fragments resulting from lagging-strand synthesis are joined via DNA ligase to form a continuous DNA molecule.

DNA helicase activity unwinds the DNA helix to expose single-stranded template DNA.

A short, complementary RNA primer is added to the template DNA strand.

Origin-recognition proteins find and bind to the origin(s) of replication.

DNA polymerase assembles at the origin and catalyzes nucleotide polymerization in the 5’ to 3’ direction.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

3.

Which of the following statements is true regarding DNA replication in prokaryotes?

The origin of replication includes a set of five binding sites for the DNA origin-recognition protein.

Telomerase activity is required to maintain the G-rich 3’ ends of chromosomes.

Bacteria undergo polymerase switching, during which the original initiating polymerase is later replaced with a more processive enzyme.

The bacterial polymerase contains both DNA and RNA synthesis activity.

Replication is initiated from multiple origins on a single, circular chromosome.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

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Chapter 28. Chapter 28

Introduction

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