Chapter 29. Chapter 29

Problem-Solving Video Assessment

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Chapter 29 Problem-Solving Video Assessment

1.

Which of the following statements about transcription initiation at standard prokaryotic promoters is true?

Transcription begins roughly 10 nucleotides downstream of the conserved TTGACA sequence.

Transcription begins roughly 10 nucleotides downstream of the conserved TATAAT sequence.

Transcription begins roughly 35 nucleotides downstream of the conserved TATAAT sequence.

Transcription begins roughly 10 nucleotides upstream of the conserved TATAAT sequence.

Transcription begins roughly 35 nucleotides upstream of the conserved TTGACA sequence.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

2.

What is the most likely transcription start site in the following prokaryotic promoter? Only the DNA coding strand is shown, and reference nucleotide positions are noted above the sequence:

DNA coding strand, with reference nucleotide positions noted above the sequence

position 52

position 50

position 43

position 39

position 32

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

3.

Consider the following prokaryotic promoters:

Promoter	-10 sequence	-35 sequence
#1	TATATT	TCGAGA
#2	TCTGAT	CTGACA
#3	TATAGT	TTGTCA
#4	TATAGA	ATGCCA
#5	TATATT	TTGACA

Table

Which is the strongest promoter?

promoter #1

promoter #2

promoter #3

promoter #4

promoter #5

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

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Chapter 29. Chapter 29

Introduction

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