You study enzyme Y and have discovered a new inhibitor of this enzyme that you would like to characterize. You perform kinetic studies in the lab, using reactions with and without 200μM inhibitor. Unfortunately, you realize after collecting your data that you forgot to label the reaction tubes, and you are uncertain which reaction contained the inhibitor. You have collected the following data:
Reaction
Vmax
Km
#1
75 μmol/min
23 μm
#2
?
?
Table
You create a double-reciprocal plot of the data from reaction #2 and find the best-fit line. The equation of this line is y = 1.20x + 0.0133.
