Introduction
Problem-Solving Video Assessment
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Problem 13.29: Counting Receptors
You have discovered a new cell-surface receptor (receptorX) that you believe binds to either insulin or epidermal growth factor (EGF). To differentiate between the two ligands, you radiolabel each ligand to a specific activity of 4 x 107 cpm per micromole. You then perform ligand–receptorX binding experiments in your cell line and generate the following counts of cell-bound radioactivity:
|
insulin |
EGF |
Radioactive ligand alone |
5563 cpm |
6012 cpm |
Radioactive ligand + 1000-fold excess unlabeled ligand |
237 cpm |
5874 cpm |
Table
Question
13.1
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_feedback_correct: Correct.
_feedback_incorrect: Incorrect.
Question
13.2
qInrN9ylXh/gwz2luDSGNLVJb5xbPVTAmZXDdk543FrGgXCd+m3iN76K6q8E37DeKQ0PSRhUmwaVz10eNDqERMf5SOPfd07tov2ekwWvbEqapx3asKujeEUAz5XPkigue/2/aaFe2TobnhJ9WTnh3XoMq91U795shRAykR9Zk52YlmCT3QILZ0qAvjJpyVQGaWB5eoHHyyVK1eHHHj8zV53AVqSN/1LOTm3BGJaHp7YeYVNZ/GFWASwfzYTsOLD/XwTuMMusxZc91wpE3URr+d0vVGxC30tNzq6RBIt7oKeauAKB1pjHxK+tOf6C1QQfAVEZXsBI2M9BbAw2MKAMQjwxYq4zYN0IUvLGTulvlhoJRII+XbBv7F1tSDJgQAHDGpUJIHL9BOEMZQLtJ7erjGdrYHjBrYUCDqqGxFHfdUYK2HhOAxwbm0P0iD6ORuwgyY4E5ChvolpjOFSCkr37UZhRMqx5S5jF68Z3Ku1pwZCEFKJab5ea8skCW2Hr1VhLzlX+4jpzPmQ+JroCBdUPhOG8wSc99OPYP11k5eH0rUG+3aXzcfAJR+S3V+4GuHDiwuEdHsrxAQwFtbrJEORET0YwVsORCtqw
_feedback_correct: Correct.
_feedback_incorrect: Incorrect.
Question
13.3
q7/HmKtekWFRl/jFNh2Zw/aTZDOw+wkIugzpnm/CCVjqmXTqJHQMX7LlnXurNZMo8yusjWRBgty06rvE79QKU5fQIBLEo6kLxSrj8iSI0m+Z7QCV6M7S7LR9qr6M5tSNlKFsZ6X26Bucnf4TxQxFlll8nJ3uCcA+Pvun+5lHwtXSuhrcHHrsKycpK68MTzm5Qmz/r8LQHAGqo19XpE1KBt97RZA5sphHVYQexbQhCZ5ym1hW8r6b48TquQOizFNUGVFS4hAa/hMBhNyh/tKeMfX/7W4FGXSFj9HCaUGW77quqCyohd8HL6qpUP7nJoE6KJTzaNvtDundqhUFu/GrIezT06vbe297
_feedback_correct: Correct.
_feedback_incorrect: Incorrect.
Question
13.4
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
_feedback_correct: Correct.
_feedback_incorrect: Incorrect.
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