Chapter 26. Problem 26.15

Problem-Solving Video Assessment

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Problem 26.15: Reductive Power

1.

Which of the following statements concerning the function of glutathione is correct?

Reduced glutathione is critical for combating oxidative stress within cells, as it oxidizes reactive oxygen species into harmless forms.

Oxidative glutathione must be regenerated following the reduction of reactive oxygen species.

NADP⁺ acts as the reducing agent in the process of regenerating GSH.

The pentose phosphate pathway, specifically glucose 6-phosphate dehydrogenase, produces the reducing agent needed in the GSH regeneration pathway.

Cells lacking functional glucose 6-phosphate dehydrogenase display elevated levels of NADPH and thus an inability to regenerate reduced glutathione.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Consider the reaction for the regeneration of reduced glutathione from oxidized glutathione using NADPH (K'_eq = 1121). Assume that the concentration of oxidized glutathione is 0.8 nM and the concentration of reduced glutathione is 15 mM.

2.

What is the concentration differential of NADPH and NADP⁺ needed to maintain these conditions?

250 times more NADPH than NADP⁺

250 times more NADP⁺ than NADPH

17,000 times more NADPH than NADP⁺

250,000 times more NADPH than NADP⁺

250,000 more NADP⁺ than NADPH

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

3.

The concentration of the reducing agent in this system is 6.5 μM. Given the information that you calculated above, what is the concentration of the oxidant in this system?

1.6 M

110.5 mM

6.5 mM

1.6 mM

26 nM

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

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Chapter 26. Problem 26.15

Introduction

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