Front Matter

Front Matter Introduction

About the Authors

Contents in Brief

Contents

Preface

Acknowledgments

Evolution, Science, and Molecular Biology

Chapter Introduction

1.1 THE EVOLUTION OF LIFE ON EARTH

1.2 HOW SCIENTISTS DO SCIENCE

KEY TERMS

ADDITIONAL READING

DNA: The Repository of Biological Information

Chapter Introduction

2.1 MENDELIAN GENETICS

2.2 CYTOGENETICS: CHROMOSOME MOVEMENTS DURING MITOSIS AND MEIOSIS

2.3 THE CHROMOSOME THEORY OF INHERITANCE

2.4 FOUNDATIONS OF MOLECULAR GENETICS

KEY TERMS

PROBLEMS

ADDITIONAL READING

Chemical Basis of Information Molecules

Chapter Introduction

3.1 CHEMICAL BUILDING BLOCKS OF NUCLEIC ACIDS AND PROTEINS

3.2 CHEMICAL BONDS

3.3 WEAK CHEMICAL INTERACTIONS

3.4 STEREOCHEMISTRY

3.5 THE ROLE OF pH AND IONIZATION

3.6 CHEMICAL REACTIONS IN BIOLOGY

KEY TERMS

PROBLEMS

ADDITIONAL READING

Protein Structure

Chapter Introduction

4.1 PRIMARY STRUCTURE

4.2 SECONDARY STRUCTURE

4.3 TERTIARY AND QUATERNARY STRUCTURES

4.4 PROTEIN FOLDING

4.5 DETERMINING THE ATOMIC STRUCTURE OF PROTEINS

KEY TERMS

PROBLEMS

ADDITIONAL READING

Protein Function

Chapter Introduction

5.1 PROTEIN-LIGAND INTERACTIONS

5.2 ENZYMES: THE REACTION CATALYSTS OF BIOLOGICAL SYSTEMS

5.3 MOTOR PROTEINS

5.4 THE REGULATION OF PROTEIN FUNCTION

KEY TERMS

PROBLEMS

ADDITIONAL READING

DNA and RNA Structure

Chapter Introduction

6.1 THE STRUCTURE AND PROPERTIES OF NUCLEOTIDES

6.2 DNA STRUCTURE

6.3 RNA STRUCTURE

6.4 CHEMICAL AND THERMODYNAMIC PROPERTIES OF NUCLEIC ACIDS

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

Studying Genes

Chapter Introduction

7.1 ISOLATING GENES FOR STUDY (CLONING)

7.2 WORKING WITH GENES AND THEIR PRODUCTS

7.3 UNDERSTANDING THE FUNCTIONS OF GENES AND THEIR PRODUCTS

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

Genomes, Transcriptomes, and Proteomes

Chapter Introduction

8.1 GENOMES AND GENOMICS

8.2 TRANSCRIPTOMES AND PROTEOMES

8.3 OUR GENETIC HISTORY

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

Topology: Functional Deformations of DNA

Chapter Introduction

9.1 CHROMOSOMES: AN OVERVIEW

9.2 DNA SUPERCOILING

9.3 THE ENZYMES THAT PROMOTE DNA COMPACTION

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

Nucleosomes, Chromatin, and Chromosome Structure

Chapter Introduction

10.1 NUCLEOSOMES: THE BASIC UNITS OF DNA CONDENSATION

10.2 HIGHER-ORDER CHROMOSOME STRUCTURE

10.3 REGULATION OF CHROMOSOME STRUCTURE

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

DNA Replication

Chapter Introduction

11.1 DNA TRANSACTIONS DURING REPLICATION

11.2 THE CHEMISTRY OF DNA POLYMERASES

11.3 MECHANICS OF THE DNA REPLICATION FORK

11.4 INITIATION OF DNA REPLICATION

11.5 TERMINATION OF DNA REPLICATION

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

DNA Mutation and Repair

Chapter Introduction

12.1 TYPES OF DNA MUTATIONS

12.2 DNA ALTERATIONS THAT LEAD TO MUTATIONS

12.3 MECHANISMS OF DNA REPAIR

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

Recombinational DNA Repair and Homologous Recombination

Chapter Introduction

13.1 RECOMBINATION AS A DNA REPAIR PROCESS

13.2 ENZYMATIC MACHINES IN BACTERIAL RECOMBINATIONAL DNA REPAIR

13.3 HOMOLOGOUS RECOMBINATION IN EUKARYOTES

13.4 NONHOMOLOGOUS END JOINING

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

DNA Mutation and Repair

Chapter Introduction

14.1 MECHANISMS OF SITE-SPECIFIC RECOMBINATION

14.2 MECHANISMS OF TRANSPOSITION

14.3 THE EVOLUTIONARY INTERPLAY OF TRANSPOSONS AND THEIR HOSTS

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

Transcription: DNA-Dependent Synthesis of RNA

Chapter Introduction

15.1 RNA POLYMERASES AND TRANSCRIPTION BASICS

15.2 TRANSCRIPTION IN BACTERIA

15.3 TRANSCRIPTION IN EUKARYOTES

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

RNA Processing

Chapter Introduction

16.1 MESSENGER RNA CAPPING AND POLYADENYLATION

16.2 PRE-mRNA SPLICING AND EDITING

16.3 RNA TRANSPORT AND DEGRADATION

16.4 PROCESSING OF NON-PROTEIN-CODING RNAs

16.5 RNA CATALYSIS AND THE RNA WORLD HYPOTHESIS

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

The Genetic Code

Chapter Introduction

17.1 DECIPHERING THE GENETIC CODE: tRNA AS ADAPTOR

17.2 THE RULES OF THE CODE

17.3 CRACKING THE CODE

17.4 EXCEPTIONS PROVING THE RULES

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

Protein Synthesis

Chapter Introduction

18.1 THE RIBOSOME

18.2 ACTIVATION OF AMINO ACIDS FOR PROTEIN SYNTHESIS

18.3 INITIATION OF PROTEIN SYNTHESIS

18.4 ELONGATION AND TERMINATION OF THE POLYPEPTIDE CHAIN

18.5 TRANSLATION-COUPLED REMOVAL OF DEFECTIVE mRNA

18.6 PROTEIN FOLDING, COVALENT MODIFICATION, AND TARGETING

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEMS

ADDITIONAL READING

Regulating the Flow of Information

Chapter Introduction

19.1 REGULATION OF TRANSCRIPTION INITIATION

19.2 THE STRUCTURAL BASIS OF TRANSCRIPTIONAL REGULATION

19.3 POSTTRANSCRIPTIONAL REGULATION OF GENE EXPRESSION

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

The Regulation of Gene Expression in Bacteria

Chapter Introduction

20.1 TRANSCRIPTIONAL REGULATION

20.2 BEYOND TRANSCRIPTION: CONTROL OF OTHER STEPS IN THE GENE EXPRESSION PATHWAY

20.3 CONTROL OF GENE EXPRESSION IN BACTERIOPHAGES

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

The Transcriptional Regulation of Gene Expression in Eukaryotes

Chapter Introduction

21.1 BASIC MECHANISMS OF EUKARYOTIC TRANSCRIPTIONAL ACTIVATION

21.2 COMBINATORIAL CONTROL OF GENE EXPRESSION

21.3 TRANSCRIPTIONAL REGULATION MECHANISMS UNIQUE TO EUKARYOTES

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEM

ADDITIONAL READING

The Posttranscriptional Regulation of Gene Expression in Eukaryotes

Chapter Introduction

22.1 POSTTRANSCRIPTIONAL CONTROL INSIDE THE NUCLEUS

22.2 TRANSLATIONAL CONTROL IN THE CYTOPLASM

22.3 THE LARGE-SCALE REGULATION OF GROUPS OF GENES

22.4 RNA INTERFERENCE

22.5 PUTTING IT ALL TOGETHER: GENE REGULATION IN DEVELOPMENT

22.6 FINALE: MOLECULAR BIOLOGY, DEVELOPMENTAL BIOLOGY, AND EVOLUTION

KEY TERMS

PROBLEMS

DATA ANALYSIS PROBLEMS

ADDITIONAL READING

Model Organisms Appendix

Appendix Introduction

Bacterium, Escherichia coli

Budding Yeast, Saccharomyces cerevisiae

Bread Mold, Neurospora crassa

Nematode, Caenorhabditis elegans

Mustard Weed, Arabidopsis thaliana

Fruit Fly, Drosophila melanogaster

House Mouse, Mus musculus

Solutions to Problems

Chapter 2

Chapter 3

Chapter 4

Chapter 5

Chapter 6

Chapter 7

Chapter 8

Chapter 9

Chapter 10

Chapter 11

Chapter 12

Chapter 13

Chapter 14

Chapter 15

Chapter 16

Chapter 17

Chapter 18

Chapter 19

Chapter 20

Chapter 21

Chapter 22

Glossary

Glossary

Index

Index

Endpaper

Endpaper Introduction