DNA cloning

cloning vector

DNA ligase

recombinant DNA

recombinant DNA technology

genetic engineering

Type II restriction endonuclease

plasmid

origin of replication (ori)

transformation

electroporation

selectable marker

screenable marker

bacterial artificial chromosome (BAC)

shuttle vector

yeast artificial chromosome (YAC)

pulsed field gel electrophoresis

DNA library

complementary DNA (cDNA)

polymerase chain reaction (PCR)

Sanger method

expression vector

transfection

site-directed mutagenesis

fusion protein

tag

green fluorescent protein (GFP)

immunofluorescence

epitope tag

tandem affinity purification (TAP) tag

yeast two-hybrid analysis

yeast three-hybrid analysis

DNA microarray