NEW AND UPDATED CONTENT

Chapter 1

• Updated discussions of evolution and the scientific method

Chapter 2

• Updated discussion of the central dogma

• Updated and expanded discussion of the types of RNA

Chapter 3

• New Moment of Discovery

• Expanded discussion of nucleosides

• Revised and expanded section: The Hydrophobic Effect Brings Together Nonpolar Molecules

• New section: Electronic Interactions between Bases in Nucleic Acids

Chapter 4

• Expanded section: Amino Acids Are Categorized by Chemical Properties

• Significantly expanded discussion of protein purification, including Highlight 4-1

• New section: Intrinsically Unstructured Proteins Have Versatile Binding Properties

• Expanded section on protein families

• Significantly expanded section on protein folding and computational biology

Chapter 5

• New Moment of Discovery

Chapter 6

• Expanded discussion of the instability of RNA

• New Highlight 6-1: DNA Nanotechnology

• New discussion of riboswitches

Chapter 7

• Expanded discussion on obtaining DNA fragments to clone

• Thoroughly updated section on next-gen and other modern DNA sequencing technologies.

• New section: Genomic Sequencing Is Aided by New Generations of DNA Sequencing Methods, incorporating the exciting new advances with programmable nucleases

Chapter 8

• Expanded Highlight 8-1, now including discussion of the microbiome

• Updated section on noncoding DNA

• Expanded section on mass spectrometry

Chapter 10

• New Moment of Discovery

• Significantly expanded discussion of histone modifications, including a new table

Chapter 11

• Expanded discussion of the β sliding clamp

• Expanded discussion of the Pol III holoenzyme

• Updated and expanded discussion of eukaryotic replication forks

• Updated and expanded section: Eukaryotic Origins “Fire” Only Once per Cell Cycle

• New section: Telomeres and Telomerase Solve the End Replication Problem in Eukaryotes

• New Highlight 11-2: Short Telomeres Portend Aging Diseases

Chapter 12

• New Moment of Discovery

• New table presenting overview of DNA repair processes

Chapter 13

• Updated and expanded sections on double-strand break repair and reconstruction of replication forks

• Updated section on meiotic recombination

Chapter 14

• Updated and expanded introductory section on transposable elements and site-specific recombination

• Updated and expanded section: Precise DNA Rearrangements Are Promoted by Site-Specific Recombinases

• Reorganized section on the use of site-specific recombination systems in biotechnology

• Updated and expanded sections on transposition

Chapter 15

• Updated section on transcription elongation

• Updated and expanded discussion of the role of transcription factors

• Updated and expanded discussion of termination mechanisms among RNA polymerases

Chapter 16

• Streamlined chapter organization

• Expanded discussion of P bodies

Chapter 18

• Streamlined chapter organization

• Updated discussion of protein release factors

• Updated discussion of nuclear export signals

Chapter 19

• Updated section: Gene Expression Is Regulated through Feedback Loops, now including inducer exclusion

Chapter 22

• Expanded section on alternative splicing, including ESEs and ESSs

• Updated section on RNA interference

• New section: RNAs Regulate a Wide Range of Cellular Processes

• Updated section on the developmental potential of stem cells