module 3 Scientific Method

Environmental indicators are important for understanding human impacts on Earth systems and the sustainability of those systems. In order to evaluate environmental indicators, we need to use reproducible scientific methods. An understanding of the scientific method is essential for environmental science.

Learning Objectives

After reading this module you should be able to

Science is a process

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Humans during the past century have learned a lot about the impact of their activities on the natural world. Scientific inquiry has provided great insights into the challenges we are facing and has suggested ways to address those challenges. For example, a hundred years ago, we did not know how significantly or rapidly we could alter the chemistry of the atmosphere by burning fossil fuels. Nor did we understand the effects of many common materials, such as lead and mercury, on human health. Much of our knowledge comes from the work of researchers who study a particular problem or situation to understand why it occurs and to determine how we can fix or prevent it from occurring. In this section we will look at the process scientists use to ask and answer questions about the environment.

The Scientific Method

Scientific method An objective method to explore the natural world, draw inferences from it, and predict the outcome of certain events, processes, or changes.

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Figure 3.1: FIGURE 3.1 The scientific method. In an actual investigation, a researcher might reject a hypothesis and investigate further with a new hypothesis, several times if necessary, depending on the results of the experiment.

To investigate the natural world, scientists, such as those who examined the effects of fracking as described at the beginning of this chapter, have to be as objective and methodical as possible. They must conduct their research in such a way that other researchers can understand how their data were collected and agree on the validity of their findings. To do this, scientists follow a process known as the scientific method, which is an objective way to explore the natural world, draw inferences from it, and predict the outcome of certain events, processes, or changes. The scientific method is used in some form by scientists in all parts of the world and is a generally accepted way to conduct science.

As we can see in FIGURE 3.1, the scientific method has a number of steps, including observing and questioning, forming hypotheses, collecting data, interpreting results, and disseminating findings.

Observing and Questioning

Homeowners and scientists noticed, in areas where fracking occurred, that certain household wells contained high methane concentrations and they wanted to know why this was occurring. Such observing and questioning is where the process of scientific research begins.

Forming Hypotheses

Hypothesis A testable conjecture about how something works.

Observing and generating questions lead a scientist to formulate a hypothesis. A hypothesis is a testable conjecture about how something works. It may be an idea, a proposition, a possible mechanism of interaction, or a statement about an effect. For example, we might hypothesize that when the air temperature rises over time, certain plant species will be more likely, and others less likely, to persist.

What makes a hypothesis testable? We can test the idea about the relationship between air temperature and plant species by growing plants in a greenhouse at different temperatures. “Fish kills are caused by something in the water” is a testable hypothesis because it speculates that there is an interaction between something in the water and the observed dead fish.

Null hypothesis A prediction that there is no difference between groups or conditions, or a statement or an idea that can be falsified, or proved wrong.

Sometimes it is easier to prove something wrong than to prove it is true beyond doubt. In this case, scientists use a null hypothesis. A null hypothesis is a prediction that there is no difference between groups or conditions, or a statement or idea that can be falsified, or proved wrong. The statement “Fish deaths have no relationship to something in the water” is an example of a null hypothesis.

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Figure 3.2: FIGURE 3.2 Accuracy and precision. Accuracy refers to how close a measured value is to the actual or true value. Precision is how close repeated measurements of the same sample are to one another.

Collecting Data

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Replication The data collection procedure of taking repeated measurements.

Sample size The number of times a measurement is replicated in data collection.

Scientists typically take several sets of measurements—a procedure called replication. The number of times a measurement is replicated is the sample size (sometimes referred to as n). A sample size that is too small can cause misleading results. For example, if a scientist chose three men out of a crowd at random and found that they all had size 10 shoes, she might conclude that all men have a shoe size of 10. If, however, she chose a larger sample size—100 men—it is very unlikely that all 100 individuals would happen to have the same shoe size.

Accuracy How close a measured value is to the actual or true value.

Precision How close the repeated measurements of a sample are to one another.

Uncertainty An estimate of how much a measured or calculated value differs from a true value.

Proper procedures yield results that are accurate and precise. They also help us determine the possible relationship between our measurements or calculations and the true value. Accuracy refers to how close a measured value is to the actual or true value. For example, an environmental scientist might estimate how many songbirds of a particular species there are in an area of 1,000 ha by randomly sampling 10 ha and then projecting or extrapolating the result up to 1,000 ha. If the extrapolation is close to the true value, it is an accurate extrapolation. Precision is how close to one another the repeated measurements of the same sample are. In the same example, if the scientist counted birds five times on five different days and obtained five results that were similar to one another, the estimates would be precise. Uncertainty is an estimate of how much a measured or calculated value differs from a true value. In some cases, it represents the likelihood that additional repeated measurements will fall within a certain range. Looking at FIGURE 3.2, we see that high accuracy and high precision is the most desirable result.

Interpreting Results

We have followed the steps in the scientific method from making observations and asking questions, to forming a hypothesis, to collecting data. What happens next? Once results have been obtained, analysis of data begins. A scientist may use a variety of techniques to assist with data analysis, including summaries, graphs, charts, and diagrams.

As data analysis proceeds, scientists begin to interpret their results. This process normally involves two types of reasoning: inductive and deductive. Inductive reasoning is the process of making general statements from specific facts or examples. If the scientist who sampled a songbird species in the preceding example made a statement about all birds of that species, she would be using inductive reasoning. It might be reasonable to make such a statement if the songbirds that she sampled were representative of the whole population. Deductive reasoning is the process of applying a general statement to specific facts or situations. For example, if we know that, in general, air pollution kills trees, and we see a single, dead tree, we may attribute that death to air pollution. But a conclusion based on a single tree might be incorrect, since the tree could have been killed by something else, such as a parasite or fungus. Without additional observations or measurements, and possibly experimentation, the observer would have no way of knowing the cause of death with any degree of certainty.

The most careful scientists always maintain multiple working hypotheses—that is, they entertain many possible explanations for their results. They accept or reject certain hypotheses based on what the data show or do not show. Eventually, they determine that certain explanations are the most likely, and they begin to generate conclusions based on their results.

Disseminating Findings

A hypothesis is never confirmed by a single experiment. That is why scientists not only repeat their experiments themselves, but also present papers at conferences and publish the results of their investigations. This dissemination of scientific findings allows other scientists to repeat the original experiment and verify or challenge the results. The process of science involves ongoing discussion among scientists, who frequently disagree about hypotheses, experimental conditions, results, and the interpretation of results. Two investigators may even obtain different results from similar measurements and experiments, as happened with investigations of fracking. Only when the same results are obtained over and over by different investigators can we begin to trust that those results are valid. In the meantime, the disagreements and discussion about contradictory findings are a valuable part of the scientific process. They help scientists refine their research to arrive at more consistent, reliable conclusions.

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Like any scientist, you should always read reports of “exciting new findings” with a critical eye. Question the source of the information, consider the methods or processes that were used to obtain the information, and draw your own conclusions. This process, essential to all scientific endeavors, is known as critical thinking.

Theory A hypothesis that has been repeatedly tested and confirmed by multiple groups of researchers and has reached wide acceptance.

A hypothesis that has been repeatedly tested and confirmed by multiple groups of researchers and has reached wide acceptance becomes a theory. Current theories about how plant species distributions change with air temperature, for example, are derived from decades of research and evidence. Notice that this sense of theory is different from the way we might use the term in everyday conversation (such as, “But that’s just a theory!”). To be considered a theory, a hypothesis must be consistent with a large body of experimental results. A theory cannot be contradicted by any replicable tests.

Scientists work under the assumption that the world operates according to fixed, knowable laws. We accept this assumption because it has been successful in explaining a vast array of natural phenomena and continues to lead to new discoveries. When the scientific process has generated a theory that has been tested multiple times, we can call that theory a natural law. A natural law is a theory to which there are no known exceptions and which has withstood rigorous testing. Familiar examples include the law of gravity and the laws of thermodynamics, which we will look at in the next chapter. These theories are accepted as facts by the scientific community, but they remain subject to revision if contradictory data are found.

Scientific Method in Action: The Chlorpyrifos Investigation

Let’s look at what we have learned about the scientific method in the context of an actual scientific investigation. In the 1990s, scientists suspected that organophosphates—a group of chemicals commonly used in insecticides—might have serious effects on the human central nervous system. By the early part of the decade, scientists suspected that organophosphates might be linked to problems such as neurological disorders, birth defects, ADHD, and palsy. One of these chemicals, chlorpyrifos (klor-PEER-i-fos), was of particular concern because it is among the most widely used pesticides in the world, with large amounts applied in homes in the United States and elsewhere.

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Figure 3.3: FIGURE 3.3 A typical experimental process. An investigation of the effects of chlorpyrifos on the central nervous system illustrates how the scientific method is used.

The Hypothesis

The researchers investigating the effects of chlorpyrifos on human health formulated a hypothesis: Chlorpyrifos causes neurological disorders and negatively affects human health. Because this hypothesis would be hard to prove conclusively, the researchers also proposed a null hypothesis: Chlorpyrifos has no observable negative effects on the central nervous system. We can follow the process of their investigation in FIGURE 3.3.

Testing the Hypothesis

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Control group In a scientific investigation, a group that experiences exactly the same conditions as the experimental group, except for the single variable under study.

To test the null hypothesis, the scientists designed experiments using rats. One experiment used two groups of rats, with 10 individuals per group. The first group—the experimental group—was fed small doses of chlorpyrifos for each of the first 4 days of life. No chlorpyrifos was fed to the second group. That second group was a control group: a group that experiences exactly the same conditions as the experimental group, except for the single variable under study. In this experiment, the only difference between the control group and the experimental group was that the control group was not fed any chlorpyrifos. By designating a control group, scientists can determine whether an observed effect is the result of the experimental treatment or of something else in the environment to which all the subjects are exposed. For example, if the control rats—those that were not fed chlorpyrifos—and the experimental rats—those that were exposed to chlorpyrifos—showed no differences in their brain chemistry, researchers could conclude that the chlorpyrifos had no effect. If the control group and experimental group had very different brain chemistry after the experiment, the scientists could conclude that the difference must have been due to the chlorpyrifos.

The Results

At the end of the experiment, the researchers found that the rats exposed to chlorpyrifos had much lower levels of the enzyme choline acetyltransferase in their brains than the rats in the control group. But without a control group for comparison, the researchers would never have known whether the chlorpyrifos or something else caused the change observed in the experimental group.

The discovery of the relationship between ingesting chlorpyrifos and a single change in brain chemistry might seem relatively small. But that is how most scientific research works: Very small steps establish that an effect occurs and, eventually, how it occurs. In this way, we progress toward a more thorough understanding of how the world works. This particular research on chlorpyrifos, combined with numerous other experiments testing specific aspects of the chemical’s effect on rat brains, demonstrated that chlorpyrifos was capable of damaging developing rat brains at fairly low doses. The results of this research have been important for our understanding of human health and toxic substances in the environment.

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Figure 3.4: FIGURE 3.4 A natural experiment. The Mount St. Helens eruption in 1980 created a natural experiment for understanding large-scale forest regrowth. (a) A pre-eruption forest near Mount St. Helens in 1979; (b) the same location, post-eruption, in 1982; (c) the same location in 2009 begins to show forest regrowth.
(U.S. Forest Service)

Controlled Experiments and Natural Experiments

The chlorpyrifos experiment we have just described was conducted in the controlled conditions of a laboratory. However, not all experiments can be done under such controlled conditions. For example, it would be difficult to study the interactions of wolves and caribou in a controlled setting because both species need large amounts of land and because their behavior changes in captivity. Other reasons that a controlled laboratory experiment may not be possible include prohibitive costs and ethical concerns.

Natural experiment A natural event that acts as an experimental treatment in an ecosystem.

Under these circumstances, investigators look for a natural experiment. A natural experiment occurs when a natural event acts as an experimental treatment in an ecosystem. For example, a volcano that destroys thousands of hectares of forest provides a natural experiment for understanding large-scale forest regrowth (FIGURE 3.4). We would never destroy that much forest just to study regrowth, but we can study such natural disasters when they occur. Still other cases of natural experiments do not involve disasters. For example, we can study the process of ecological succession by looking at specific areas where forests have been growing for different amounts of time and comparing them. We can study the effects of species invasions by comparing uninvaded ecosystems with invaded ones.

Because a natural experiment is not controlled, many variables can change at once, and results can be difficult to interpret. Ideally, researchers compare multiple examples of similar systems in order to exclude the influences of different variables. For example, after a forest fire, researchers might not only observe how a burned forest responds to the disturbance but also compare it with a nearby forest that did not burn. In this case, the researchers are comparing similar forests that differ in only one variable, fire. If, however, they tried to compare the burned forest with a different type of forest, perhaps one at a different elevation, it would be difficult to separate the effects of the fire from the effects of elevation. Still, because they may be the only way to obtain vital information, natural experiments are indispensable.

Let us return to the study of chlorpyrifos. Researchers wanted to know if human brains that were exposed to the chemical would react in the same way as rat brains. Because researchers would never feed pesticides to humans to study their effects, for obvious ethical reasons, they conducted a natural experiment. They looked for groups of people who were similar in most ways—for example, income, age, level of education—but who varied in their exposure to chlorpyrifos. To gather data on variation of exposure they looked at how often people in each group used pesticides that contained the chemical, the brand they used, and the frequency and location of use. Researchers found that tissue concentrations of chlorpyrifos were highest in groups that were exposed to the chemical in their jobs and among poor urban families whose exposure to residential pesticides was high. Among these populations, a number of studies connected exposure to chlorpyrifos with low birth weight and other developmental abnormalities.

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Science and Progress

The chlorpyrifos experiment is a good example of the process of science. Based on observations, the scientists proposed a hypothesis and null hypothesis. The null hypothesis was tested and rejected. Multiple rounds of additional testing gave researchers confidence in their understanding of the problem. Moreover, as the research progressed, the scientists informed the public, as well as the scientific community, about their results. Finally, in 2000, as a result of the step-by-step scientific investigation of chlorpyrifos, the U.S. Environmental Protection Agency (EPA) decided to prohibit its use for most residential applications. It also prohibited agricultural use on fruits that are eaten without peeling, such as apples and pears, and those that are especially popular with children, such as grapes.

Environmental science presents unique challenges

Environmental science has many things in common with other scientific disciplines. However, it presents a number of challenges and limitations that are not usually found in most other scientific fields. These challenges and limitations are a result of the nature of environmental science and the way research in the field is conducted.

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Figure 3.5: FIGURE 3.5 The global nature of human impacts. The trash that washed up onto the beach of this remote Pacific island vividly demonstrates the difficulty of finding any part of Earth unaffected by human activities.
(Ashley Cooper/Alamy)

Lack of Baseline Data

The greatest challenge to environmental science is the fact that there is no undisturbed baseline—no “control planet”—with which to compare contemporary Earth. Virtually every part of the globe has been altered by humans in some way (FIGURE 3.5). Even though some remote regions appear to be undisturbed, we can still find quantities of lead in the Greenland ice sheet, traces of the anthropogenic compound PCB in the fatty tissue of penguins in Antarctica, and invasive species from many locations carried by ship to remote tropical islands. This situation makes it difficult to know the original levels of contaminants or numbers of species that existed before humans began to alter the planet. Consequently, we can only speculate about how the current conditions deviate from those of prehuman activity.

Subjectivity

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A second challenge unique to environmental science lies in the dilemmas raised by subjectivity. For example, when you go to the grocery store, the bagger may ask, “Paper or plastic?” How can we know for certain which type of bag has the least environmental impact? There are techniques for determining what harm may come from using the petrochemical benzene to make a plastic bag and from using chlorine to make a paper bag. However, different substances tend to affect the environment differently: Benzene may pose more of a risk to people, whereas chlorine may pose a greater risk to organisms in a stream. It is difficult, if not impossible, to decide which is better or worse for the environment overall. There is no single measure of environmental quality. Ultimately, our assessments and our choices involve value judgments and personal opinions.

Interactions

A third challenge is the complexity of natural and human-dominated systems. All scientific fields examine interacting systems, but those systems are rarely as complex and as intertwined as they are in environmental science. Because environmental systems have so many interacting parts, the results of a study of one system cannot always be easily applied to similar systems elsewhere.

There are also many examples in which human preferences and behaviors affect environmental systems as much as the natural laws that describe them. For example, many people assume that if we built more efficient automobiles, the overall consumption of gasoline in the United States would decrease. To decrease gas consumption, however, it is necessary not only to build more efficient automobiles, but also to get people to purchase those vehicles and use them in place of less efficient ones. During the 1990s and early 2000s, even though there were many fuel-efficient cars available, the majority of buyers in the United States continued to purchase larger, heavier, and less fuel-efficient cars, minivans, light trucks, and sport-utility vehicles. Environmental scientists thought they knew how to reduce gasoline consumption, but they neglected to account for consumer behavior.

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Figure 3.6: FIGURE 3.6 Electronic waste recycling. The poor are exposed to a disproportionate amount of pollutants and other hazards. The people shown here, located in a small village on the outskirts of New Delhi, India, are recycling circuit boards from discarded electronics products.
(Peter Essick/Aurora Photos/Alamy)

Human Well-Being

As we continue our study of environmental science, we will see that many of its topics touch on human well-being. In environmental science, we study how humans impact the biological systems and natural resources of the planet. We also study how changes in natural systems and the supply of natural resources affect humans.

We know that people who are unable to meet their basic needs are less likely to be interested in or able to be concerned about the state of the natural environment. The principle of environmental equity—the fair distribution of Earth’s resources—adds a moral issue to questions raised by environmental science. Pollution and environmental degradation are inequitably distributed, with the poor receiving much more than an equal share. Is this a situation that we, as fellow humans, can tolerate? Environmental justice is a social movement and field of study that works toward equal enforcement of environmental laws and the elimination of disparities, whether intended or unintended, in how pollutants and other environmental harms are distributed among the various ethnic and socioeconomic groups within a society (FIGURE 3.6).