Producing cDNA molecules with sticky ends

Adding EcoRI sites to the ends of cDNA molecules. The cDNA molecules come from the last step in Figure 10-4. Adapters (boxed region) are added at both ends of the cDNA molecules. These adapters are double-stranded oligonucleotides that contain a restriction site (EcoRI is shown in red) and random DNA sequence at both ends (represented by N).