Detecting rearrangements by their altered hybridization signals

To detect chromosomal rearrangements, mutant and wild-type genomic DNA is tagged with dyes that fluoresce at different wavelengths. These tagged DNAs are added to cDNA clones arranged in chromosomally ordered microarrays, and the ratio of bound fluorescence at each wavelength is calculated for each clone. The expected results for a normal genome and three types of mutants are illustrated.