Table 20-4: Molecular Substitutions in Two ϕ-X174 Bacteriophages, TX and ID, During Adaptation

Order*	TX site	Amino acid change	ID site	Amino acid change
1	782	E72, T → 1	2167	F388, H → Q
2	1727	F242, L → F	1613	F204, T → S
3	2085	F361, A → V	1533⁶	F177, T → I
4	319	C63, V → F	1460	F153, Q → E
5	2973	H15, G → S	1300	F99, silent
6	323	C64, D → G	1305³	F101, G → D
7	4110³	A44, H → Y	1308	F102, Y → C
8	1025	F8, E → K	4110¹	A44, H → Y
9	3166⁷	H79, A → V	4637	A219, silent
10	5185	A402, T → M	965-91⁴	deletion
11	1305²	F101, G → D	5365⁵	A462, M → T
12	965-91⁴	deletion	4168⁷	A63, Q → R
13	5365⁵	A462, M → T	3166²	H79, A → V
14	1533¹	F177, T → I	1809	F269, K → R
15	4168⁶	A63, Q → R
*Changes are listed in the order in which they appeared in each of the two bacteriophage selection lines. The nucleotide position is listed, followed by the protein affected, A–H, with the number of the amino acid residue and the nature of the amino acid substitution. Parallel changes are shown in boldface, and a superscript indicates the order of those changes in the other virus selection line.
Source: H. A. Wichman et al., Science 285, 1999, 422–424.