This example from yeast shows the initiation of DNA synthesis at an origin of replication in a eukaryote. As with prokaryotic initiation (see Figure 7-20), proteins of the origin recognition complex (ORC) bind to the origin, where they separate the two strands of the double helix and recruit replisome components at the two replication forks. Replication is linked to the cell cycle through the availability of two proteins: Cdc6 and Cdtl.