Frontmatter

Book Title

About the Authors

Introduction

Contents in Brief

Contents

Preface

Acknowledgments

The Genetics Revolution

Chapter Introduction

1.1 The Birth of Genetics

1.2 After Cracking the Code

1.3 Genetics Today

SUMMARY

KEY TERMS

PROBLEMS

Single-Gene Inheritance

Chapter Introduction

2.1 Single-Gene Inheritance Patterns

2.2 The Chromosomal Basis of Single-Gene Inheritance Patterns

2.3 The Molecular Basis of Mendelian Inheritance Patterns

2.4 Some Genes Discovered by Observing Segregation Ratios

2.5 Sex-Linked Single-Gene Inheritance Patterns

2.6 Human Pedigree Analysis

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

APPENDIX 2-1 Stages of Mitosis

APPENDIX 2-2 Stages of Meiosis

Independent Assortment of Genes

Chapter Introduction

3.1 Mendel's Law of Independent Assortment

3.2 Working with Independent Assortment

3.3 The Chromosomal Basis of Independent Assortment

3.4 Polygenic Inheritance

3.5 Organelle Genes: Inheritance Independent of the Nucleus

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

Mapping Eukaryote Chromosomes by Recombination

Chapter Introduction

4.1 Diagnostics of Linkage

4.2 Mapping by Recombinant Frequency

4.3 Mapping with Molecular Markers

4.4 Centromere Mapping with Linear Tetrads

4.5 Using the Chi-square Test to Infer Linkage

4.6 Accounting for Unseen Multiple Crossovers

4.7 Using Recombination-Based Maps in Conjunction with Physical Maps

4.8 The Molecular Mechanism of Crossing Over

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

The Genetics of Bacteria and Their Viruses

Chapter Introduction

5.1 Working with Microorganisms

5.2 Bacterial conjugation

5.3 Bacterial Transformation

5.4 Bacteriophage Genetics

5.5 Transduction

5.6 Physical Maps and Linkage Maps compared

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

Gene Interaction

Chapter Introduction

6.1 Interactions Between the Alleles of a single gene: Variations on Dominance

6.2 Interaction of Genes in Pathways

6.3 Inferring Gene Interactions

6.4 Penetrance and Expressivity

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

DNA: Structure and Replication

Chapter Introduction

7.1 DNA: The Genetic Material

7.2 DNA Structure

7.3 Semiconservative Replication

7.4 Overview of DNA Replication

7.5 The Replisome: A Remarkable Replication Machine

7.6 Replication in Eukaryotic Organisms

7.7 Telomeres and Telomerase: Replication Termination

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

RNA: Transcription and Processing

Chapter Introduction

8.1 RNA

8.2 Transcription

8.3 Transcription in Eukaryotes

8.4 Intron Removal and Exon Splicing

8.5 Small Functional RNAs That Regulate and Protect the Eukaryotic Genome

SUMMARY

KEY TERMS

PROBLEMS

Proteins and Their Systhesis

Chapter Introduction

9.1 Protein Structure

9.2 The Genetic Code

9.3 tRNA: The Adapter

9.4 Ribosomes

9.5 The Proteome

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

Gene Isolation and Manipulation

Chapter Introduction

10.1 Overview: Isolating and Amplifying Specific DNA Fragments

10.2 Generating Recombinant DNA Molecules

10.3 Using Molecular Probes to Find and Analyze a Specific Clone of Interest

10.4 Determining the Base Sequence of a DNA Segment

10.5 Aligning Genetic and Physical Maps to Isolate Specific Genes

10.6 Genetic Engineering

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

Regulation of Gene Expression in Bacteria and Their Viruses

Chapter Introduction

11.1 Gene Regulation

11.2 Discovery of the lac System: Negative Control

11.3 Catabolite Repression of the lac Operon: Positive Control

11.4 Dual Positive and Negative Control: The Arabinose Operon

11.5 Metabolic Pathways and Additional Levels of Regulation: Attenuation

11.6 Bacteriophage Life Cycles: More Regulators, Complex Operons

11.7 Alternative Sigma Factors Regulate Large Sets of Genes

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

Regulation of Gene Expression in Eukaryotes

Chapter Introduction

12.1 Transcriptional Regulation in Eukaryotes: An Overview

12.2 Lessons from Yeast: The GAL System

12.3 Dynamic Chromatin

12.4 Activation of Genes in a Chromatin Environment

12.5 Long-Term Inactivation of Genes in a Chromatin Environment

12.6 Gender-Specific Silencing of Genes and Whole Chromosomes

12.7 Post-Transcriptional Gene Repression by miRNAs

SUMMARY

KEY TERMS

SOLVED PROBLEMS

The Genetic Control of Development

Chapter Introduction

13.1 The Genetic Approach to Development

13.2 The Genetic Toolkit for Drosophila Development

13.3 Defining the Entire Toolkit

13.4 Spatial Regulation of Gene Expression in Development

13.5 Post-transcriptional Regulation of Gene Expression in Development

13.6 From Flies to Fingers, Feathers, and Floor Plates: The Many Roles of Individual Toolkit Genes

13.7 Development and Disease

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

Genomes and Genomics

Chapter Introduction

14.1 The Genomics Revolution

14.2 Obtaining the Sequence of a Genome

14.3 Bioinformatics: Meaning from Genomic Sequence

14.4 The Structure of the Human Genome

14.5 The Comparative Genomics of Humans with Other Species

14.6 Comparative Genomics and Human Medicine

14.7 Functional Genomics and Reverse Genetics

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

The Dynamic Genome: Transposable Elements

Chapter Introduction

15.1 Discovery of Transposable Elements in Maize

15.2 Transposable Elements in Prokaryotes

15.3 Transposable Elements in Eukaryotes

15.4 The Dynamic Genome: More Transposable Elements Than Ever Imagined

15.5 Regulation of Transposable Element Movement by the Host

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

Mutation, Repair, and Recombination

Chapter Introduction

16.1 The Phenotypic Consequences of DNA Mutations

16.2 The Molecular Basis of Spontaneous Mutations

16.3 The Molecular Basis of Induced Mutations

16.4 Biological Repair Mechanisms

16.5 Cancer: An Important Phenotypic Consequence of Mutation

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

Large-Scale Chromosomal Changes

Chapter Introduction

17.1 Changes in Chromosome Number

17.2 Changes in Chromosome Structure

17.3 Overall Incidence of Human Chromosome Mutations

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

Population Genetics

Chapter Introduction

18.1 Detecting Genetic Variation

18.2 The Gene-Pool Concept and the Hardy-Weinberg Law

18.3 Mating Systems

18.4 Genetic Variation and Its Measurement

18.5 The Modulation of Genetic Variation

18.6 Biological and Social Applications

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

The Inheritance of Complex Traits

Chapter Introduction

19.1 Measuring Quantitative Variation

19.2 A Simple Genetic Model for Quantitative Traits

19.3 Broad-Sense Heritability: Nature Versus Nurture

19.4 Narrow-Sense Heritability: Predicting Phenotypes

19.5 Mapping QTL in Populations with Known Pedigrees

19.6 Association Mapping in Random-Mating Populations

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

Evolution of Genes and Traits

Chapter Introduction

20.1 Evolution by Natural Selection

20.2 Natural Selection in Action: An Exemplary Case

20.3 Molecular Evolution: The Neutral Theory

20.4 Cumulative Selection and Multistep Paths to Functional Change

20.5 Morphological Evolution

20.6 The Origin of New Genes and Protein Functions

SUMMARY

KEY TERMS

SOLVED PROBLEMS

PROBLEMS

A Brief Guide to Model Organisms

A Brief Guide to Model Organisms

Escherichia coli

Saccharomyces cerevisiae

Neurospora crassa

Arabidopsis thaliana

Caenorhabditis elegans

Drosophila melanogaster

Mus musculus

Appendix

Appendix A Genetic Nomenclature

Appendix B Bioinformatic Resources for Genetics and Genomics

Endpaper

Index to Model Organisms

A Map of Genetics

Answers to Selected Problems

Chapter Introduction

Chapter 2

Chapter 3

Chapter 4

Chapter 5

Chapter 6

Chapter 7

Chapter 8

Chapter 9

Chapter 10

Chapter 11

Chapter 12

Chapter 13

Chapter 14

Chapter 15

Chapter 16

Chapter 17

Chapter 18

Chapter 19

Chapter 20