KEY TERMS

amplification

antibody

autoradiogram

bacterial artificial chromosome (BAC)

cDNA library

chromosome walk

complementary DNA (cDNA)

dideoxy (Sanger) sequencing

DNA cloning

DNA ligase

DNA palindrome

DNA technology

donor DNA

ectopically

fine mapping

fosmid

functional complementation (mutant rescue)

gel electrophoresis

gene knockout

gene replacement

genetically modified organism (GMO)

genetic engineering

genomic library

genomics

hybridization

mutant rescue (functional complementation)

Northern blotting

polymerase chain reaction (PCR)

positional cloning

position effect

probe

recombinant DNA

restriction enzyme

restriction fragment

RNA blotting

Sanger (dideoxy) sequencing

Southern blotting

Ti plasmid

transgene

transgenic organism

vector