Chapter
1. 10.3 Finding Specific Cloned Genes by Functional Complementation: Making a Library of Wild-Type Yeast DNA
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10.3 Finding Specific Cloned Genes by Functional Complementation: Making a Library of Wild-Type Yeast DNA
1.1
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Question 1 of 3
Questions
Question
1.1
Which of the following is NOT an essential part of a bacterial plasmid that will be used to complement a mutant yeast strain? (NOTE: a selectable marker for yeast needs to be in the plasmid as well.)
A.
B.
C.
D.
E.
Correct.
Incorrect.
2
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Question
1.2
Why is genomic DNA from a wild-type (WT) yeast strain used for library construction?
A.
B.
C.
D.
E.
Correct.
Incorrect.
2
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Question
1.3
Why is genomic DNA digested into 2-10 kb fragments?