Chapter
1. 10.5 Finding Specific Cloned Genes by Functional Complementation: Using the Cloned GAL Gene as a Probe for GAL and mRNA
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10.5 Finding Specific Cloned Genes by Functional Complementation: Using the Cloned GAL Gene as a Probe for GAL and mRNA
1.1
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Question 1 of 2
Questions
Question
1.1
Below is a list of steps for performing a Northern blot. Place them in the correct order.
1. Size fractionate RNA by gel electrophoresis 2. Expose membrane to X-ray film 3. Wash off probe that is not bound by complementary base pairing. 4. Isolate cellular RNA 5. Incubate membrane with radioactive GAL gene 6. Grow yeast in galactose or glucose medium 7. Transfer RNA to an absorbent membrane by capillary action
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Correct.
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Question
1.2
The GAL gene is transcribed into mRNA when yeast cells are grown on galactose. What type of role might the GAL gene have?