Chapter
1. 10.6 SDS Gel Electrophoresis and Immunoblotting
Animated Art
10.6 SDS Gel Electrophoresis and Immunoblotting
1.1
Animated Art
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Animation Assessment
30
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Question 1 of 3
Questions
Question
1.1
Which of the following is NOT a function of SDS during SDS-PAGE?
A.
B.
C.
D.
E.
Correct.
Incorrect.
2
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Question
1.2
After SDS-PAGE, the gel is placed next to nitrocellulose. Where will the + charge need to be to get the proteins to migrate to the nitrocellulose? Why is nitrocellulose used?
A.
B.
C.
D.
E.
Correct.
Incorrect.
2
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Question
1.3
A larger protein will bind more of the anionic SDS detergent than a smaller protein, yet it migrates more slowly through the polyacrylamide network toward the + electrode. Why doesn’t the protein with more negative charge move faster toward the + electrode?