Chapter
1. 12.3 Gal4 Modularity and Gene Induction in Non-Yeast Species
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12.3 Gal4 Modularity and Gene Induction in Non-Yeast Species
1.1
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Question 1 of 5
Questions
Question
1.1
Why is the gene encoding jellyfish GFP used as a reporter?
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Question
1.2
A gal3- mutant harbors the GFP reporter construct. All other components of the GAL regulation system are present. When will GFP be expressed at detectable levels?
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Question
1.3
A gal80- mutant harbors the GFP reporter construct. All other components of the GAL regulation system are present. When will GFP be expressed at detectable levels?
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Question
1.4
The DNA consensus sequence for LexA is located next to the GFP reporter instead of the GAL4 UAS. Which transcription factor will activate high levels of GFP? (DBD = DNA binding domain and AD = activation domain.)
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Question
1.5
In Drosophila, a construct is introduced in which GAL4 is placed near a wing-specific promoter. A GAL4 UAS with a minimal promoter is placed next to the GFP reporter and also introduced into Drosophila. In which tissue will GAL4 be expressed? In which tissue will GFP be observed?