Chapter
1. 14.1 DNA Microarrays: Using an Oligonucleotide Array to Analyze Patterns of Gene Expression
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14.1 DNA Microarrays: Using an Oligonucleotide Array to Analyze Patterns of Gene Expression
1.1
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Question 1 of 3
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Question
1.1
The purpose of a microarray experiment is to:
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Correct.
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Question
1.2
mRNA is isolated from roots and leaves. The root mRNA is copied into cDNA and labeled with a green fluorescent dye while the leaf mRNA is copied into cDNA and labeled with a red fluorescent dye. After hybridization to an oligonucleotide microarray, a laser is used to excite the array and two emission wavelengths are measured separately. Oligonucleotide spots for genes expressed in leaves, but not roots, will be ____________, whereas oligonucleotide spots for genes expressed in roots, but not leaves, will be ____________. Oligonucleotide spots for genes expressed equally in both tissues will be ____________.
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Correct.
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Question
1.3
A researcher isolates mRNA from normal and cancer cells and, by mistake, adds the same fluorescence dye to both samples during cDNA synthesis. What will be the outcome of the microarray?