Chapter
1. 5.1 Bacterial Conjugation and Mapping by Recombination
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5.1 Bacterial Conjugation and Mapping by Recombination
1.1
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Question 1 of 7
Questions
Question
1.1
During conjugation, pili attach to ____________ cells and ____________-stranded DNA is transferred through a pore. The first DNA to enter the recipient cell is the ____________.
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Correct.
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Question
1.2
The _____________ DNA cannot replicate and is lost when the E. coli cell divides.
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Correct.
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Question
1.3
A(n) ____________ number of crossovers are required to maintain a ____________ bacterial genome.
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Correct.
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Question
1.4
After conjugation is complete, recombinants are selected on a medium containing streptomycin, but lacking leucine in order to select for:
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Correct.
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Question
1.5
The gene that enters the recipient strain ____________ is the gene that is ____________ selected.
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Question
1.6
The following recombinants are recovered when conjugation occurs between an a+d+g+ donor and an a-d-g- recipient. a+ d+ g+ = 84% a- d- g+ = 6% a+ d- g+ = 10% a- d+ g+ = less than 1% Which gene is in the middle?
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Correct.
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Question
1.7
The following recombinants are recovered when conjugation occurs between an a+d+g+ donor and an a-d-g- recipient. a+ d+ g+ = 84% a- d- g+ = 6% a+ d- g+ = 10% a- d+ g+ = less than 1% What is the map distance between the a and d genes?