In DNA, the strand defined as the coding strand is the one with the same sequence as the mRNA that is eventually derived from that segment of DNA. The sequence shown in the BRCA2 sequence file is that of the coding strand (by convention). Also by convention, that sequence is represented in the 5' to 3' direction. The nucleotide provided as a starting point should thus serve to orient your sequence from top to bottom.
Section 1: Refer to Figure 8-4 for the difference between deoxyribonucleotides and ribonucleotides. Refer to Figure 8-11 for base-pairing rules.
Section 2: Refer to Figure 8-17. Section 3: Refer to Figure 8-16.
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Overalyed!!
BUILD THE DNA HERE
Coding Strand
Template Strand
BUILD THE RNA HERE
Congratulations, you have properly constructed the DNA sequence of this portion of the BRCA2 gene. Now build the mRNA that would be transcribed from the template strand of the DNA. Start by placing the top ribonucleotide and work your way down.
Great! You have properly constructed the mRNA sequence for this portion of the BRCA2 gene and have successfully completed this portion of the exercise. Click continue to proceed.
mRNA
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Prerequisite skills needed:
Nucleotide Structure
Relevant book section: pages 279-289
By completing this simulation, you will:
Understand the important structural features of DNA and RNA polynucleotides
Understand the tertiary structure of a DNA molecule
Build DNA and RNA strands
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Toggle the buttons below to classify each structure as A-DNA, B-DNA, or Z-DNA, and also indicate the handedness of each structure. When you are finished, click the Submit button.
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Examine these sugar pucker conformations. In which form of DNA is each typically found? Toggle the buttons to select either A-DNA or B-DNA, then click the Submit button.