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Click on the attacking nucleophile.
Great—now click on the atom it attacks.
Simulating....
DNA Pol senses the incorrect base pairing, and is stalled. Click on the activity that will proofread the incorrect nucleotide.
Click on the attacking nucleophile.
Great! Now click on the molecule it is attacking.

Prerequisite skills needed:

  • Nucleotide Structure
  • DNA Structure and Base Pairing
  • Relevant book section: pages 369-376

By completing this simulation, you will:

  • Understand the DNA polymerase reactions
  • Conduct nucleophilic attack to form a phosphodiester bond between nucleotides
  • Use the exonuclease to correct incorporation of a mismatched nucleotide