Metagenomics allows us to describe new organisms and ecosystems

If you take a microbiology laboratory course you’ll learn how to identify various prokaryotes on the basis of their growth on particular artificial media. For example, staphylococci are a group of bacteria that infect skin and nasal passages. When grown on a medium called blood agar, they form round, raised colonies. Microorganisms can also be identified by their nutritional requirements or the conditions under which they will grow (for example, aerobic versus anaerobic). Such culture methods have been the mainstay of microbial identification for more than a century and are still useful and important. However, scientists can now use PCR and DNA sequencing to identify microbes without culturing them in the laboratory.

In 1985 Norman Pace, then at Indiana University, came up with the idea of isolating DNA directly from environmental samples. He used PCR to amplify specific rRNA-encoding sequences from the samples to determine whether particular microbes were present. The PCR products were sequenced to explore their diversity. The term metagenomics was coined to describe this approach of analyzing genes without isolating the intact organism. It is now possible to sequence DNA samples from almost any environment. The sequences can be used to detect the presence of both known microbes and heretofore unidentified organisms (Figure 17.3). For example:

*connect the concepts Learn more about how disruptions of the human microbiome are associated with various autoimmune diseases in Key Concept 25.3.

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Figure 17.3 Metagenomics Microbial DNA extracted from the environment can be sequenced and analyzed. This has led to the description of many new genes and species.

These and other discoveries are truly extraordinary and potentially very important. It is estimated that 90 percent of the microbial world has been invisible to biologists and is only now being revealed by metagenomics. Entirely new ecosystems of bacteria and viruses are being discovered in which, for example, one species produces a molecule that another metabolizes. It is hard to overemphasize the importance of such an increase in our knowledge of the hidden world of microbes. This knowledge will help us understand natural ecological processes, and has the potential to help us find better ways to manage environmental catastrophes such as oil spills, or to remove toxic heavy metals from soil.

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