Plants produce constitutive chemical defenses against herbivores

Plants attract, resist, and inhibit other organisms with a wide range of chemicals known as secondary metabolites. Primary metabolites are substances such as proteins, nucleic acids, carbohydrates, lipids, and their building blocks, which are produced and used by all living organisms, including plants. Primary metabolites are used in basic cellular processes such as photosynthesis, respiration, and nutrient uptake. Secondary metabolites are substances that are not used for basic cellular processes. Each is found in only certain organisms or groups of organisms.

The more than 10,000 known plant secondary metabolites range in molecular mass from about 70 to more than 380,000 daltons, but most have a low molecular mass (Table 38.1). The effects of defensive secondary metabolites on animals are diverse. Some act on the nervous systems of herbivorous insects, mollusks, or mammals. Others mimic the natural hormones of insects, causing some larvae to fail to develop into adults. Still others damage the digestive tracts of herbivores. Some secondary metabolites are toxic to fungal pathogens. Many secondary metabolites are not just used for defense; they have other roles in the plant. For instance, the phenolics called anthocyanins are toxic to insects and bacteria. But they also are pigmented and aid in attracting pollinators, accumulate in vacuoles and so contribute to osmotic pressure in cells, and even regulate dormancy in some plants.

table 38.1 Secondary Metabolites Used in Defense
Class Type Role Example

Nitrogen-containing

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Alkaloids Neurotoxin Nicotine in tobacco
Glycosides Inhibit electron transport Dhurrin in sorghum
Nonprotein amino acids Disrupt protein structure Canavanine in jack bean

Nitrogen–sulfur-containing

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Glucosinolates Inhibit respiration Methylglucosinolate in cabbage

Phenolics

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Coumarins Block cell division Umbelliferone in carrots
Flavonoids Phytoalexins Capsidol in peppers
Tannins Inhibit enzymes Gallotannin in oak trees

Terpenes

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Monoterpenes Neurotoxins Pyrethrin in chrysanthemums
Diterpenes Disrupt reproduction and muscle function Gossypol in cotton
Triterpenes Inhibit transport Digitalis in foxglove
Sterols Block animal hormones Spinasterol in spinach
Polyterpenes Deter feeding Latex in Euphorbia

Here is an example of how one of these metabolites works on pathogens. Canavanine is a secondary metabolite whose role is defensive and is based on its chemical structure. Canavanine is an amino acid that is not normally found in proteins, but is very similar to the amino acid arginine, which is found in almost all proteins.

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When an insect larva consumes canavanine-containing plant tissue, the canavanine is incorporated into the insect’s proteins in some of the places where the mRNA codes for arginine, because the enzyme that charges the tRNA specific for arginine fails to discriminate accurately between the two amino acids (see Key Concept 14.5). The structure of canavanine, however, is different enough from that of arginine that some of the resulting proteins end up with a modified tertiary structure, and hence reduced biological activity. These defects in protein structure and function lead to developmental abnormalities that kill the insect.

Taking cues from nature, we have practical uses for many plant secondary metabolites or molecules made from them in the lab. Throughout human history, these have formed the basis of herbal medicine as plant extracts are used. As knowledge of chemistry accumulated, people purified the active ingredients of these plants. For instance, the foxglove plant gave us the cardiac drug digitalis.