In this simulation, you were able to use a PCR technique to analyze patient samples to specify which pathogens had infected which patients. Which component used in the PCR procedure was responsible for the specificity of this analysis?
A.
B.
C.
D.
Correct. See Key Concept 13.5 The Polymerase Chain Reaction Amplifies DNA
Incorrect. See Key Concept 13.5 The Polymerase Chain Reaction Amplifies DNA
Activity 13.2 Quiz
Prior to the introduction of DNA polymerase from the thermophilic bacteria Thermus aquaticus, DNA polymerase had to be introduced into the reaction fresh every cycle. Why was this the case, and what is unique about Taq (Thermus aquaticus) DNA polymerase?
A.
B.
C.
D.
Correct. See Key Concept 13.5 The Polymerase Chain Reaction Amplifies DNA
Incorrect. See Key Concept 13.5 The Polymerase Chain Reaction Amplifies DNA
Activity 13.2 Quiz
Refer to the figure below.
The figure shows the results of a PCR analysis testing the presence of three pathogens in patient samples. Would you recommend that this set of tests be repeated and handled in a different way?
A.
B.
C.
D.
Correct. See Key Concept 13.5 The Polymerase Chain Reaction Amplifies DNA
Incorrect. See Key Concept 13.5 The Polymerase Chain Reaction Amplifies DNA
Activity 13.2 Quiz
Assuming you have four copies of your desired template DNA to start, and you run a 25-cycle PCR, how many copies of DNA will you have at the end of the PCR (under optimal conditions)?
A.
B.
C.
D.
Correct. See Key Concept 13.5 The Polymerase Chain Reaction Amplifies DNA
Incorrect. See Key Concept 13.5 The Polymerase Chain Reaction Amplifies DNA
