Genetic Analysis of Mutations to Identify and Study Genes
Adams, A. E. M., D. Botstein, and D. B. Drubin. 1989. A yeast actin-
Griffiths, A. G. F., et al. 2000. An Introduction to Genetic Analysis, 7th ed. W. H. Freeman and Company.
Guarente, L. 1993. Synthetic enhancement in gene interaction: a genetic tool comes of age. Trends Genet. 9:362–
Hartwell, L. H. 1967. Macromolecular synthesis of temperature-
Hartwell, L. H. 1974. Genetic control of the cell division cycle in yeast. Science 183:46.
Nüsslein-
Simon, M. A., et al. 1991. Ras1 and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase. Cell 67:701–
Tong, A. H., et al. 2001. Systematic genetic analysis with ordered arrays of yeast deletion mutants. Science 294:2364–
DNA Cloning and Characterization
Ausubel, F. M., et al. 2002. Current Protocols in Molecular Biology. Wiley.
Gubler, U., and B. J. Hoffman. 1983. A simple and very efficient method for generating cDNA libraries. Gene 25:263–
Han, J. H., C. Stratowa, and W. J. Rutter. 1987. Isolation of full-
Itakura, K., J. J. Rossi, and R. B. Wallace. 1984. Synthesis and use of synthetic oligonucleotides. Annu. Rev. Biochem. 53:323–
Maniatis, T., et al. 1978. The isolation of structural genes from libraries of eucaryotic DNA. Cell 15:687–
Nasmyth, K. A., and S. I. Reed. 1980. Isolation of genes by complementation in yeast: molecular cloning of a cell-
Nathans, D., and H. O. Smith. 1975. Restriction endonucleases in the analysis and restructuring of DNA molecules. Annu. Rev. Biochem. 44:273–
Roberts, R. J., T. VIncze, J. Posfai, and D. Macelis. 2015. REBASE—
Using Cloned DNA Fragments to Study Gene Expression
Andrews, A. T. 1986. Electrophoresis, 2d ed. Oxford University Press.
Erlich, H., ed. 1992. PCR Technology: Principles and Applications for DNA Amplification. W. H. Freeman and Company.
Pellicer, A., et al. 1978. The transfer and stable integration of the HSV thymidine kinase gene into mouse cells. Cell 41:133–
Saiki, R. K., et al. 1988. Primer-
Sanger, F. 1981. Determination of nucleotide sequences in DNA. Science 214:1205–
Souza, L. M., et al. 1986. Recombinant human granulocyte-
Wahl, G. M., J. L. Meinkoth, and A. R. Kimmel. 1987. Northern and Southern blots. Method. Enzymol. 152:572–
Wallace, R. B., et al. 1981. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit β-globin DNA. Nucleic Acids Res. 9:879–
Locating and Identifying Human Disease Genes
Botstein, D., et al. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Genet. 32:314–
Donis-
Hartwell, L., et al. 2006. Genetics: From Genes to Genomes. McGraw-
Hastbacka, T., et al. 1994. The diastrophic dysplasia gene encodes a novel sulfate transporter: positional cloning by fine-
Orita, M., et al. 1989. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5:874.
Tabor, H. K., N. J. Risch, and R. M. Myers. 2002. Opinion: candidate-
Inactivating the Function of Specific Genes in Eukaryotes
Capecchi, M. R. 1989. Altering the genome by homologous recombination. Science 244:1288–
Deshaies, R. J., et al. 1988. A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides. Nature 332:800–
Fire, A., et al. 1998. Potent and specific genetic interference by double-
Gu, H., et al. 1994. Deletion of a DNA polymerase beta gene segment in T cells using cell type-
Jinek, M., et al. 2012. A programmable dual-
Zamore, P. D., et al. 2000. RNAi: double-
Zimmer, A. 1992. Manipulating the genome by homologous recombination in embryonic stem cells. Annu. Rev. Neurosci. 15:115.