Chapter 3. SDS Gel Electrophoresis

Animation Activities

true

false

Animation Activity 3-38: SDS Gel Electrophoresis

1.

In SDS-polyacrylamide gel electrophoresis, proteins are resolved based on their:

charge.

molecular weight.

total negative charge.

shape and charge.

shape.

Correct.

Incorrect.

Try Again.

2.

SDS coats proteins with _________________ charges.

negative

positive

neutral

cationic

None of the above is correct.

Correct.

Incorrect.

Try Again.

3.

Which statement is false?

When a protein sample containing SDS is heated, the SDS molecules bind the proteins and cause them to fold into their native state.

When a protein sample containing SDS is heated, the SDS molecules bind to the proteins and cause them to denature.

SDS coats the proteins with a uniform negative charge.

Proteins treated with SDS have a similar shape and charge-to-mass ratio.

All of the above statements are true.

Correct.

Incorrect.

Try Again.

4.

Polypeptides treated with SDS have similar charge-to-mass ratios; therefore, the distance they travel through a gel is dependent:

only on charge.

only on shape.

only on molecular weight.

on shape and charge.

only on the total positive charge.

Correct.

Incorrect.

Try Again.

Activity results are being submitted...

Chapter 3. SDS Gel Electrophoresis

Introduction

1.

2.

3.

4.