Chapter 3. Immunoblotting
Introduction
Animation Activities
true
false
Animation Activity 3-41: Immunoblotting
1.
Put the following immunoblotting steps in the correct order:
1. A chemiluminescent reaction is performed to detect bound antibody.
2. A secondary antibody binds to the primary antibody.
3. A primary antibody binds to the protein of interest.
4. The nitrocellulose membrane is coated with an inert protein such as albumin.
5. The proteins are transferred from the gel to the blot using electrophoresis.
| A. |
| B. |
| C. |
| D. |
| E. |
Correct.
Incorrect.
Try Again.
2
2.
When using the immunoblotting technique, the primary antibody detects:
| A. |
| B. |
| C. |
| D. |
| E. |
Correct.
Incorrect.
Try Again.
2
3.
When using the immunoblotting technique, the secondary antibody detects:
| A. |
| B. |
| C. |
| D. |
| E. |
Correct.
Incorrect.
Try Again.
2
4.
Enzyme-linked secondary antibodies allow detection of:
| A. |
| B. |
| C. |
| D. |
| E. |
Correct.
Incorrect.
Try Again.
2
Activity results are being submitted...