Chapter 4. Preparing Monoclonal Antibodies

Animation Activities

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Animation Activity: 4-6: Preparing Monoclonal Antibodies

1.

Which can produce a single type of antibody specific for a single molecular determinant?

B lymphocytes

T lymphocytes

phagocytic cells

macrophages

All of the above can produce a single type of antibody specific for a single molecular determinant.

Correct.

Incorrect.

Try Again.

2.

Which statement is false?

Protein X will bind to B lymphocytes that express its specific antibodies.

A mouse spleen contains B lymphocytes with differing specificities.

The first step in preparing monoclonal antibodies specific to Protein X is to inject a mouse with a solution of Protein X.

When Protein X binds a B lymphocyte, a series of events is triggered that stimulates the cell to grow and divide.

Protein molecules injected into a mouse will reach the liver, where they encounter B lymphocytes.

Correct.

Incorrect.

Try Again.

3.

Fusion of a myeloma cell with a spleen cell results in:

monoclonal antibodies.

hybridomas.

polyclonal antibodies.

non-specific lymphocytes.

malignant tumor cells.

Correct.

Incorrect.

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4.

Hybridomas:

are immortal.

are HGPRT+.

are able to survive in HAT medium.

can be used to produce monoclonal antibodies.

All of the above are correct regarding hybridomas.

Correct.

Incorrect.

Try Again.

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Chapter 4. Preparing Monoclonal Antibodies

Introduction

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