Chapter 4. FRET & FRET Biosensors

Animation Activities

true

false

Animation Activity 4-24 and 4-25: FRET & FRET Biosensors

1.

FRET is based on:

the distance between two DNA molecules.

the distance between two protein molecules.

the conformations of two protein molecules.

the phosphorylation status of a protein.

the presence of a ligand and sensor domain within a protein.

Correct.

Incorrect.

Try Again.

2.

If Protein X is fused to CFP and Protein Y to YFP within a cell, an interaction between the two proteins can be assumed if:

fluorescence is detected at 530 nm.

fluorescence is detected at 433 nm.

fluorescence is detected at 475 nm.

phosphorylation is detected in both Proteins X and Y.

a green color is seen under a fluorescence microscope.

Correct.

Incorrect.

Try Again.

3.

FRET will not occur between two proteins that are more than _______ apart.

10 nm

10 µm

10 mm

All of the above are correct.

None of the above is correct.

Correct.

Incorrect.

Try Again.

4.

If the FRET biosensor example provided here is used to determine whether kinase or phosphatase activity is favored in a cellular environment, which statement is true?

Detection of fluorescence at 475nm would suggest phosphatase activity is favored.

Detection of fluorescence at 530nm would suggest phosphatase activity is favored.

Detection of fluorescence at 433nm would suggest kinase activity is favored.

Detection of fluorescence at 530nm would suggest that neither activity is occurring.

Binding of the ligand domain to the sensor domain to trigger energy transfer would suggest phosphatase activity is occurring.

Correct.

Incorrect.

Try Again.

Activity results are being submitted...

Chapter 4. FRET & FRET Biosensors

Introduction

1.

2.

3.

4.