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DNA Cloning in a Plasmid Vector

Chapter 6. DNA Cloning in a Plasmid Vector

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Animation Activity: 6-14: DNA Cloning in a Plasmid Vector

1.

Which is a method for isolating a particular sequence of DNA from a complete mixture of different DNA sequences?

DNA cloning

RNA cloning

SDS-PAGE

Western blot analysis

All of the above could be used to isolate a particular sequence of DNA from a complete mixture of different DNA sequences.

Correct.

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2.

Which is NOT found in a plasmid vector?

a cloning site

a drug-resistance gene

a replication origin

the genome of the host cell

All of the above are found in a plasmid vector.

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3.

Which is used to cleave a vector at the cloning site?

a palindromic DNA sequence (GGAATTTC)

PCR

a foreign piece of DNA

a restriction enzyme

a single-stranded piece of DNA

Correct.

Incorrect.

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4.

What is the role of the enzyme DNA ligase?

to hybridize the sticky ends of the foreign DNA and the plasmid DNA

to seal the sticky ends of the foreign DNA and the plasmid DNA via phosphodiester linkages

to infer ampicillin resistance

to create a library of recombinant plasmids

DNA ligase is responsible for all of the above.

Correct.

Incorrect.

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5.

Which process is used to insert recombinant plasmids into host cells such as E. coli bacteria?

SDS-PAGE

PCR

transformation

antibody staining

gel electrophoresis

Correct.

Incorrect.

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Chapter 6. DNA Cloning in a Plasmid Vector

Introduction

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