Chapter 6. The Polymerase Chain Reaction

Animation Activities

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Animation Activity 6-18: The Polymerase Chain Reaction

The purpose of the polymerase chain reaction (PCR) is to:

amplify the number of copies of a DNA segment of interest.

decrease the number of copies of an RNA segment of interest.

decrease the number of copies of a DNA segment of interest.

eliminate a specific number of copies of a DNA segment of interest in a cell.

amplify the number of copies of an RNA segment of interest.

Correct.

Incorrect.

Try Again.

Which of the following is/are required for the polymerase chain reaction (PCR) to occur?

Taq polymerase

target DNA

nucleotides

DNA primers

All of the above are required for the PCR to occur.

Correct.

Incorrect.

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Taq polymerase:

is derived from hot springs bacteria.

can tolerate the high temperatures used in a PCR reaction.

functions optimally at 72°C.

begins polymerization after the primers anneal.

All of the above are correct regarding Taq polymerase.

Correct.

Incorrect.

Try Again.

After three cycles of PCR, how many copies of the double-stranded target DNA sequence exist?

Correct.

Incorrect.

Try Again.

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